biological indicator spore testing procedure

Many are due to some type of human error. A second failure indicates the likelihood of a mechanical or procedural problem.Ī number of factors may be involved with a sterilization failure. However, the spore test should be immediately repeated. If operational (for example, time, temperature, and pressure) and chemical (internal and external) indicators suggest that the sterilizer is functioning properly, then a single spore strip failure likely does not indicate sterilizer malfunction. In such cases, proper processing and interpretation of the results must be carefully completed. Some practices use monitoring systems that allow in-house incubation. The list of questions in the box at right is to be asked of the practice's compliance coordinator and to the practice's monitoring service in the event of a positive spore test. A review of the monitoring process would be useful. Failure to kill the spores (a 'positive' test, bacterial growth) is a significant event that requires immediate action. The results of a 'negative' test indicate sterilization. The desired outcome of biological testing is the killing of the spore challenge.

At least weekly spore testing is recommended.

Also, spore testing is an essential part of the evaluation of any repaired sterilizer prior to placing it back into use. This is especially valuable when new types of packaging are selected or when different loading procedures are employed. Monitoring evaluates both sterilizer and office personnel performance and the materials that are used. Regular use of spore tests helps to support the primary goal of sterilization - patient safety through the universal delivery of sterile instruments chairside. Biological indicators (BI) are the most valid method for monitoring the sterilization process.

Biological indicator spore testing procedure